TRANSCRIPT PROFILING OF CANDIDA ALBICANS STRAINS LACKING OR OVEREXPRESSING THE APSES PROTEINS Efg1p AND Efh1p
These data have been published in Doedt T, Krishnamurthy S, Bockmuhl DP, Tebarth B, Stempel C, Russell CL, Brown AJ, Ernst JF. APSES proteins regulate morphogenesis and metabolism in Candida albicans. Mol Biol Cell 15(7):3167-80 (2004).
|
Species |
Candida albicans |
|
Strains |
wt: CAF2-1
(Fonzi
& Irwin, 1993) wt: CAI4
(Fonzi
& Irwin, 1993) efg1: HLC67
(Lo
et al., 1997) efh1: C4/d6-3 (this work) efg1-efh1: H/1.26
(this work) PCK1p-EFH1 : CAI4 transformed with pDB35 PCK1p-EFG1 : CAI4 transformed with pRC2312-P-H PCK1p- : CAI4 transformed
with pBI-1 |
|
|
Noninducing conditions: Strains were
cultured in YPD-medium at 30¡C to an OD600= 0.5 Hyphae-inducing
conditions: Strains were
cultured in YPD-medium at 30¡C to an OD600= 0.5, than washed in
water and resuspended in YP-medium containing 10 % serum and than
incubated additional 30 minutes at 37¡C Overexpresssing
Strains: Strains were
cultured in SCAA -medium at 30¡C to an OD600= 0.5 |
|
RNA obtention |
by dropping of
the cell samples into liquid N2 and trizol extraction, as described
(Murad et al., 2001) |
|
labeling |
Direct labelling
with Cy3/C5-dCTP as described here |
|
Targets |
Cy3/Cy5-labelled cDNA |
|
Microchip features |
C. albicans glass microarrays
with probes for 5907 genes as described here |
|
Microchip manufacturer |
|
|
Hybridization method |
As described here |
|
Imaging & Data capture |
Fuji (FLA-8000);
AIDA Array Metrix (Raytest) |
|
Normalisation method |
Intensity-dependent
method (Lowess) applied to the print-tip (GeneSpring v 5.0) |
|
Quality analysis |
Detection of differentially
expressed genes: SAM-software as described here
with indicated parameters and an addional minimal 1.5-fold change |
Download
all txt-files
Data
(non inducing conditions)
|
experiment |
Cy3 labelling |
Cy5 labelling |
Date of hybridization |
Slide |
Name of Cy3-file |
Name of Cy5-file |
Name of Genespring-file |
||||
|
efg1:wt |
CAF2-1 |
HLC52 |
07/10/02 |
12 |
19-532.txt |
19-640.txt |
19.ZIP |
||||
|
|
HLC52 |
CAF2-1 |
09/13/02 |
19 |
35-532.txt |
35-640.txt |
35.ZIP |
||||
|
|
CAF2-1 |
HLC52 |
09/13/02 |
20 |
39-532.txt |
39-640.txt |
39.ZIP |
||||
|
efh1:wt |
CAF2-1 |
C4/d6-3 |
07/10/02 |
11 |
16-532.txt |
16-640.txt |
16.ZIP |
||||
|
|
C4/d6-3 |
CAF2-1 |
09/13/02 |
9 |
34-532.txt |
34-640.txt |
lign:center">C4/d6-3
09/13/02 |
10 |
36-532.txt |
36-640.txt |
36.ZIP |
|
efg1-efh1:wt |
CAF2-1 |
H/1.26 |
07/10/02 |
13 |
15-532.txt |
15-640.txt |
15.ZIP |
||||
|
|
CAF2-1 |
H/1.26
|
09/13/02 |
17 |
38-532.txt |
38-640.txt |
38.ZIP |
||||
|
|
H/1.26
|
CAF2-1 |
09/13/02 |
18 |
37-532.txt |
37-640.txt |
37.ZIP |
Data
(hypha-inducing conditions)
|
experiment |
Cy3 labelling |
Cy5 labelling |
Date of hybridization |
Slide |
Name of Cy3-file |
Name of Cy5-file |
Name of Genespring-file |
||||
|
efg1:wt |
CAF2-1 |
HLC52 |
05/08/03 |
13 |
062_532 |
062_640 |
Induziert
efg (62).ZIP |
||||
|
|
HLC52
ss="MsoNormal" align="center" style="text-align:center">069_532 |
069_532 |
Induziert
efg (69).ZIP |
||||||||
|
|
HLC52 |
CAF2-1 |
06/06/03 |
19 |
073_532 |
073_640 |
Induziert
efg (73).ZIP |
||||
|
efh1:wt |
CAF2-1 |
C4/d6-3 |
05/08/03 |
14 |
063_532 |
063_640 |
Induziert
efh (63).ZIP |
||||
|
|
C4/d6-3 |
CAF2-1 |
06/05/03 |
17 |
C4/d6-3 |
CAF2-1 |
06/06/03 |
20 |
074_532 |
074_640 |
Induziert
efh (74).ZIP |
|
efg1-efh1:wt |
CAF2-1 |
H/1.26 |
05/08/03 |
15 |
064_532 |
064_640 |
Induziert
doppel (64).ZIP |
||||
|
|
H/1.26 |
CAF2-1 |
06/05/03 |
18 |
071_532 |
071_640 |
Induziert
doppel (71).ZIP |
||||
|
|
H/1.26 |
CAF2-1 |
06/06/03 |
21 |
075_532 |
075_640 |
Induziert
doppel (75).ZIP |